What information is not given by a restriction map?
Restriction maps give information about the lengths of DNA between the restriction sites. They do not show any information about the DNA sequences of the fragments.
Where are restriction sites located?
Restriction sites , or restriction recognition sites , are located on a DNA molecule containing specific (4-8 base pairs in length) sequences of nucleotides, which are recognized by restriction enzymes .
Which of the following sequences are most likely to be recognized by a restriction enzyme?
most restriction enzymes recognize a “perfect” palindrome , Hence GTCGAC(the complimentary sequence is CAGCTG ) sequence is most likely to be a restriction enzyme recognization site, So, the correct answer is “GTCGAC’.
What do restriction enzymes use to identify sites on DNA?
Restriction enzymes are found in bacteria (and other prokaryotes). They recognize and bind to specific sequences of DNA , called restriction sites . When it finds its target sequence, a restriction enzyme will make a double-stranded cut in the DNA molecule.
What is a DNA restriction map?
Restriction mapping is a method used to map an unknown segment of DNA by breaking it into pieces and then identifying the locations of the breakpoints. This method relies upon the use of proteins called restriction enzymes, which can cut, or digest, DNA molecules at short, specific sequences called restriction sites .
What is the difference between Type 1 and Type 2 restriction enzymes?
Today, scientists recognize three categories of restriction enzymes : type I , which recognize specific DNA sequences but make their cut at seemingly random sites that can be as far as 1,000 base pairs away from the recognition site; type II , which recognize and cut directly within the recognition site; and type III,
What do you notice about each restriction site?
What do you notice about each restriction site ? What does the word palindrome mean? Each restriction site explains more about DNA sequences, proteins, A palindrome is a word, phrase, number, or other sequence of characters which read the same backwards or forwards.
What is a Type 2 restriction enzyme?
Type II enzymes cut DNA at defined positions close to or within their recognition sequences. They produce discrete restriction fragments and distinct gel banding patterns, and they are the only class used in the laboratory for routine DNA analysis and gene cloning.
How do you identify a palindrome sequence?
For a nucleotide sequence to be considered as a palindrome , its complementary strand must read the same in the opposite direction . For example, the sequence 5′-CGATCG-3′ is considered a palindrome since its reverse complement 3′-GCTAGC-5′ reads the same. Palindromes can be exact or approximate.
Which of the following two enzymes are needed to construct a cDNA library?
(g) The following enzymes are needed to make a cDNA library : DNA polymerase, DNA ligase, RNA polymerase, restriction enzymes . False. All the enzymes are needed except RNA polymerase. Two kinds of DNA polymerase are used.
Which lane shows a digest with only the restriction enzyme BamHI?
3. Which lane shows a digest with BamHI only ? BamHI would make cuts at the three BamHI restriction sites, producing DNA fragments of 2kB, 6kB, and 12kB (4kb + 8kb). Therefore, Gel lane III shows the digest with BamHI only .
How many types of restriction endonucleases are there?
Restriction enzymes are traditionally classified into four types on the basis of subunit composition, cleavage position, sequence specificity and cofactor requirements.
Which enzyme is used to cut the DNA?
Restriction enzymes , found naturally in bacteria , can be used to cut DNA fragments at specific sequences, while another enzyme, DNA ligase , can attach or rejoin DNA fragments with complementary ends.
Which type II restriction enzyme cuts the methylated DNA?
Type II restriction endonucleases (REases) are sequence-specific endonucleases that recognize short DNA sequences and cut the DNA at defined positions within or close to the recognition sequence . In the producer cell, the host DNA is protected by specific methylation of the recognition sequence .